In the early 80’ when I was working for a government sub-contractor (that built electronics for the military, NASA, etc.) I ran into a study that was commissioned by the Air Force on the re-use of nylon locking nuts. The study was to determine if Nylock nuts could ever be reused and if it could, how many times it could be re-used.
I can’t remember the details of the study, but what I recall is that a Nylock nut did retain its locking feature for a number of re-use.
The study involved a statistical analysis of a large sample testing since griping torque of any individual nut varies even when they are new.
The bottom line, as I remember, is that you could easily reuse a Nylock about half a dozen times (min., possibly more like 10 to 20 times) and be safely within its specified tolerance for griping torque. As I recall the biggest issue they had is how to keep track the number of reuse or re-qualify (test, etc.) them for reuse.
Keep in mind that this study was to see if Nylock nuts could be reused in the field…in a high vibration environment…for combat aircrafts, etc.
I feel very safe reusing a Nylock several times, but I can easily tell as I screw one in if it feels too loose.