I think what you're asking is whether the size and morphology (shape/structure) of the PFO influence the amount of shunting; if so, then definitely yes. As you pointed out, some are so small or tight that they don't shunt at all. Others shunt only with increased intrathoracic pressure, and others will shunt at rest. The Valsalva maneuver that divers are taught to perform in order equalize their middle ear spaces (with apologies in advance to
@Angelo Farina ) is different than the Valsalva maneuver used to increase intrathoracic pressure when testing for PFO. The latter involves bearing down as if trying to have a bowel movement. Hopefully nobody is trying to clear their ears that vigorously.
PFO testing involves injecting some form of ultrasound contrast material, either sterile 0.9% saline solution that's been agitated to create microbubbles or a manufactured product. The contrast will appear in the right side of the heart soon after it's injected. The sonographer will look for shunting to the left side of the heart at rest, then will ask the patient to bear down and look for shunting with Valsalva as described above. When papers on PFO mention bubble grades, they're talking about the microbubbles in the agitated saline that have shunted during testing. Higher bubble grades mean a more robust shunt.
Best regards,
DDM