I think you missed my point.
I'm saying to check the mV readings with the unit closed - i.e. just prior to pulling a negative. Make sure they are stable. It doesn't matter what is in the loop (air or trimix).
Then you pull a negative and check the mV readings.
Then let it sit and check the readings again.
The only thing that matters is how much the readings changed in that few minutes. So, the only "aging" of the sensors that comes into play is the 5 minutes (or however long) of that actual test.
In my test, I had air inside the loop and my sensors showed 10.3mV. I sucked a negative down to 9.3mV.
If you do the same thing, you might have TX10/50 inside your loop. Before you pull the negative, you sensors might be showing 6.1mV. After you pull the negative, you might be seeing 5.4mV. Using my proposal of 1% of the original value means you'd be looking for a change of 0.06mV or less. Assuming the computer only shows a resolution of 0.1mV, that means you'd be looking to see that your sensors did not go above 5.5mV during the negative test.
And yes, I have a rEvo. If your JJ doesn't need the same kind of test, then, well, this kind of test is not for you.