@stuartv the sensors are designed for a 42 month life in 100% O2 at ambient pressure. 10 extra minutes a day in O2 vs air is going to do **** all to their longevity.
Also there are no "CCR engineers" most of these things were garage designed and don't put anything into the checklists more than it's a list of stuff you have to do in a reasonably logical order. I.e. there is no strong reason to do O2 or Dil first when analyzing the mixes.
Why is the unit full of dil at the end of the build? What is the downside of doing dil first and having O2 in the loop at the end of the build right before you splash?
On the cells being cold. I was building my unit right around New Years in cave country when it was near freezing and the head was outside which caused the cells to be really low and sluggish. If I had calibrated during assembly I would have killed myself on the next dive. Knowing not to calibrate it was critical and the cells were perfectly fine in the water. Passed their 1.6 checks at 20ft and dil was spot on at 100ft. The same applies to really warm/wet cells that may act a bit weird, though the membranes are a lot better than they used to be on the moisture front and if you're doing that, then you may want to calibrate after breathing on the unit for 10-20 minutes when everything has stabilized in order to give some predictability to how the cells are going to behave if you're doing a huge dive the next day.
You can't account for any of the environmental factors when calibrating though which is scary, so that's why I teach/preach/believe that you should only calibrate when you need to. On that trip over New Years, the unit was actually still on the calibration from Thanksgiving believe it or not.
Overwriting it is not a problem on any of the Shearwater units in terms of risk of screwing it up, but on the old Megs it was hugely problematic because they had to be 2-point calibrated which is a righteous PITA, though admittedly quite useful to have, but it's also just extra button presses that aren't necessary. I can't tell you how many times I have had to restart the calibration process on the Meg because it wasn't perfectly stabilized in air and it messed the 2-point up.