I build my rEvo either way. Whatever is convenient during the build. Cylinders are ready to go before the scrubber is built up, they go on first. Sometimes I will be building the rEvo not knowing the site yet and will wait to know what cylinders to put on, in which case the scrubber is fully built first.
I ended up making a new set of checklist cards for my rEvo. Once I added the transmitters and running a NERD instead of dreams, it wasn't a very good checklist anymore. So I rewrote them and get a them laser engraved, just like the originals. One thing I moved way up on the pre-jump list was opening the O2. Had an almost incident where I was working the checklist and had the DIL open, working on something that paused the checklist, looked down and saw a stupid PPO2 number. The checklist now goes; open dil, check ADV (for those that still run it hooked up), open O2. Now you can go through the rest of the list and have some sort of O2 being added while doing so.