@Rose Robinson in terms of "how long" it depends on the unit, but I would not expect them to hold for the expected duration of a dive. The rebreathers are not pressurized during the dive, they remain at ambient pressure. The positive and negative pressure checks are there to find leaks and they should hold for about 5 minutes. Some units hold positive and negative better than others. The Meg does not hold particularly well because it uses a compression fit sleeve for the DSV so you can't create as much of a vacuum or as much pressure as you would normally and this is where you need to know your unit and how it behaves.
@Zebra 1 On the EAC discussion. You aren't going to overbreathe a scrubber, IF the unit has passed CE rating and you aren't some superhuman. What people call overbreathing the scrubber is actually outbreathing your lungs. What causes people to THINK they have overbreathed a rebreather is CO2 retention in the lungs due to the higher work of breathing which causes the feeling of not being able to breathe and symptoms of hypercapnia.
The WOB tests for CE require breathing rates of 40lpm which is around 1.5cfm. Most people can't maintain that for any length of time, but the rebreathers are all tested to 3cfm anyway which no normal person can maintain for any length of time without passing out.
Definitions used for the ~1.5cfm test
A physically fit diver, taking slow deep breaths while swimming hard,
can sustain an RMV of 37.5 lpm for a few minutes.
For the 3cfm test
A diver athlete in superb physical condition, doing severe work,
can sustain an RMV of 75 lpm for one or two minutes.
For the extendair testing done by NEDU
Dive Rite O2ptima Rebreather Scrubber Duration Test Results | Dive Gear Express®