Adjustable Center to Center distance manifolds with right hand and left hand threads are in effect a turnbuckle, turning the Iso will eventually change the Center to center distance, but only after you account for lash in the threads.
I like to verify that I have the manifold adjusted correctly after assembling doubles by checking that I can still turn the Iso with modest effort. If I cannot move it all by hand, somethings wrong, and if I can turn it 1/4 to may be 1/2 turn with ~500-1000 psi, all is good. Pump em full and things will get stiffer. Tanks change diameter with pressure, which impacts C to C distances and the orings will try to squeeze out of any clearances available.
I leave the jam nuts loose, and have never had an Iso change orientation unexpectedly.
Tobin