BACKGROUND: The D1 dopamine receptor, expressed in several nephron segments, participates in the regulation of water and electrolyte transport. Because the renal D1 receptor is desensitized in genetic hypertension, we sought to determine the mechanism(s) of the desensitization of D1 receptors endogenously expressed in renal proximal tubules. METHODS: The mechanisms involved in the homologous desensitization of the D1 receptor in human renal proximal tubule cells were studied by measuring the production of cAMP in response to stimulation or inhibition of G protein-coupled receptor kinase (GRK) activity and expression. Protein expression was assessed by immunoblotting.
^^ hey cool, I typed that in 2 hrs ago!
^^ hey cool, I typed that in 2 hrs ago!