The above is NOT correct and gets argued ad nauseum. Cylinders are either O2 compatible or not - regardless of whether one is filling via banked NITROX or mixing via introducing pure O2. The standard is any cylinder containing 23% or greater O2 must be O2 compatible.
Here is one the better explanations:
Aluminum scuba cylinders in oxygen-enriched and oxygen service - Luxfer: Setting The Standard Worldwide
How clean does my cylinder actually need to be for oxygen service?
It needs to be oxygen cleanwhich means free from contaminants that can be the fuel for an oxygen-fed fire (see the list of contaminants in Question 3, above). Cleanliness on surfaces is measured by milligrams (mg) of hydrocarbon per square foot of surface area (mg/ft2). In the U.S., DOT (in RRC 901c) requires that the cleanliness of cylinders used in oxygen service be proven to be less than 2.5 mg/ft2, which is a level considerably below what can be seen by the naked eye. For this reason, a visual inspection after cleaning is not enough. A test must be performed to verify that the required cleanliness level has been achieved. In CFR49 173.302(b), DOT specifies an approved cleaning method and a standard test to determine the cleanliness of cylinders.
While many people argue about whether the correct threshold for O2 cleaning is 40% or 23.5%, the people who argue for 23.5% based on the CFR always seem to ignore part of that law. The CFR calls for the cylinder to be tested IAW
RR-C-901.
"Each DOT cylinder must be cleaned in accordance with the requirements of GSA Federal Speci- fication RRC901D, paragraphs 3.3.1 and 3.3.2 (IBR, see §171.7 of this sub- chapter). Cleaning agents equivalent to those specified in Federal Specification RRC901D may be used provided they do not react with oxygen.
One cylinder selected at random from a group of 200 or fewer and cleaned at the same time must be tested for oil contamination in accordance with Federal Specification RRC901D, paragraph 4.3.2, and meet the specified standard of cleanliness."
"4.3.2 Oil and hydrocarbon residue. Place a clean plug, cork, or rubber stopper in the cylinder neck, clean the area around it thoroughly with heptafluorocyclopentane (HFCPA) solvent, and wipe dry with a clean cloth. For cylinders with less than three square feet of internal surface area, remove the plug and pour in 300 milliliters (mL) of the solvent. For larger cylinders, add an additional 100 mL for each square foot of internal area over 3 square feet. Replace the plug and lay the cylinder on its side. Roll or rotate through 360 degrees on a level surface or level rolling/tumbling machine for a minimum of 10 minutes to assure all the internal surfaces have been thoroughly wetted with solvent. Remove the solvent extract into a clean beaker. Any undissolved liquid floating on the surface of the solvent would indicate the presence of water or glycerin. The solvent extract shall be analyzed for hydrocarbons by one of the following methods:
4.3.2.1 Evaporation method. Evaporate the extract to dryness at slightly below the boiling point and finish the drying in an oven at 221 oF (105 oC) ±1.8 oF (1 oC) for 15 minutes. Cool, weigh, and report as milligrams of extracted oil. All traces of solvent shall be removed from the cylinder upon completion of this test. Nonconformance to the requirements paragraph 3.3.2 shall constitute failure of this test.
4.3.2.2 Infrared spectrophotometer. A sample of the solvent extract shall be analyzed against a reference standard of the base solvent with a known hydrocarbon level of 2.5 mg per 100 mL. A response in fractional range displaying a greater contamination of hydrocarbons in the solvent extract than found in the reference standard of 2.5 mg per 100 mL shall constitute failure of this test. All traces of solvent shall be removed from the cylinder upon completion of this test."
None of the diveshops that have quoted the 23.5% rule to me have ever been able to show that performed the test required by that regulation, they just dropped a UV light in the tank and visually inspected it just like everyone else. I have yet to meet anyone who claims to follow the 23.5% rule "because it is required by federal regulation" that also performs the test required by those same regulations.