I have used balanced regs almost exclusively for the last 2 decades. Occasionally I test dive an unbalanced second and I definitely notice the difference even when they are tuned for optimum performance.
The balanced design potentially allows large orifices and lighter springs whihc both equate to better response, flow rate and inhalation effort.
On the other hand, some divers like their balanced regs set up to breathe a little harder for personal reasons that have always eluded me and/or made no sense. For those divers a balanced second stage is in most cases a waste of money.
I would also argue that an unbalanced second stage will breathe better on a balanced first stage than on an unbalanced first stage due to the stable IP delivered by the balanced design. It is able to be tuned to a lighter inhalation effort and can then deliver peak performance as the IP does not change.
Similarly, a balanced second stage will deliver noteably superior performance compared to an unbalanced second stage when used with an unbalanced first stage as the balanced second stage design levels out much (but not all) of the effects of the change in IP on inhalation effort that you would normally experience with an unbalanced second stage.
So in effect, if you add a balanced second stage to a unbalanced Mk 2, you'll notice a big improvement in inhalation effort. If you add a balanced second stage to a balanced frst stage like the Mk 17 or Mk 25, you will note a lot less improvement (and perhaps very little improvement) IF the orignal unbalanced second stage had been tuned to deliver optimum performance and minimum cracking effort.